The biological revolutions of computationally designed proteins, induced pluripotent stem cells (iPSCs), and the CRISPR-Cas9 system finally enables modifications that can spur deep understanding of spatial requirement of epigenetic information. This commentary describes the utility of a computationally designed protein, EED Binder (EB), when fused to dCas9 (EBdCas9) for identifying critical sites of PRC2 dependent histone H3K27me3 marks in the chromatin. By using EBdCas9 and gRNA, PRC2 function can be inhibited at specific loci, resulting in precise reduction of EZH2 and H3K27me3 marks, and in some (but not all) locations, activation of the gene and functional outcomes (such as regulation of cell cycle or trophoblast transdifferentiation).

Functional proteomics of seminal plasma has been reviewed by Samanta and co-workers, highlighting greater biologic relevancy and diverse functions of this seminal component to reproductive biology than previously thought. Although not requested for successful fertilization, important biological functions are associated with seminal plasma and the authors suggested that selected proteins may serve as diagnostic biomarkers for various conditions.

In many metazoan tissues, highly specialized cells are constantly lost and need to be replaced by tissue homeostasis from adult stem cells. When adult stem cells divide by mitosis their daughter cells either become new stem cells, or enter a proliferation and differentiation path. Recent studies in Drosophila males showed that germline stem cells (GSCs) can increase their mitotic activity in response to repeated mating.

Sickness rates of children living in regions with extreme environmental pollution resulted in our study evaluating effects of 30-day supplementation with two different food supplements containing nucleotides, amino acids and polypeptides or β-glucan. This study was based on published studies evaluating these effects on children with chronic respiratory problems transported to the region without environmental pollution and into the medical program complemented with climatotherapy, speleotherapy, higher motion activity, rehabilitation, and a special nutritional program. 

In the previous study, Park et al. reported the efficacy of a single-cell protein manufactured from a growth-accelerated high-vital Corynebacterium glutamicum strain and potential strain selection strategy for development of non-GMO industrial strains harboring enriched target nutritional component, named as growth?acceleration?targeted evolution (G.A.T.E.) strategy [1].