In our previous study, we synthesized a novel Fluorinated Trifluoromethyl 4-Thiazolidinone (FTT), which demonstrated promising in vitro anti-cancer activity against Ovarian cancer cells (SKOV3) and Cervical cancer cells (HeLa cells). Hence in the present work, we further investigated it’s probable genotoxic potentials on normal cells in vitro using Chinese Hamster Ovary cells (CHO-K1). Based on the IC50 value in CHO-K1 (4.53 μM), three sub-lethal concentrations were chosen (1, 1.5 and 2 μM) and different cytogenetic toxicity endpoints like induction of chromosome aberrations (CAs), micronuclei (MN) formation, Mitotic index (MI) and Cell cycle studies were performed. The results revealed that, FTT at all the concentrations induced a statistically significant increase in the induction of percentage of aberrant metaphases (P< 0.001, mitotic index (P< 0.001) and induced a lesser percentage of micronuclei compared to vehicle control. A higher percentage of cells in the G1 phase of the cell cycle indicates cell death by FTT occurred may be due to apoptosis mechanism. These preliminary toxicological data establish FTT toxicity profile, providing a fundamental understanding of its safety that is required for further development as a novel anticancer drug.

4-Thiazolidinone, CHO-K1, Cytogenetic toxicity