Degradation of Caffeine, artificially impregnated in sterile and unsterile soil, studied under ambient conditions using the Himalayan psychrotolerant Pseudomonas sp. (GBPI_Hb5). The caffeine degradation was found increased significantly in presence of GBPI_Hb5. The t_1/2 value was estimated during kinetic study of caffeine degradation both in the presence and absence of bacteria in sterilized and unsterilized soil samples, which was also found reduced significantly. 

Biodegradation, Caffeine, Psychrotolerant bacteria, Himalayas, Soil