Commentary Open Access
Volume 2 | Issue 1 | DOI: https://doi.org/10.46439/signaling.2.041
Sample multiplexing in CyTOF: Path to optimize single-cell proteomic profiling
Muharrem Muftuoglu1,*, Michael Andreeff1
- 1Section of Molecular Hematology and Therapy, Department of Leukemia, The University of Texas MD Anderson Cancer Center, Houston, TX, 77030, USA
Corresponding Author
Muharrem Muftuoglu, mmuftuoglu@mdanderson.org
Received Date: June 06, 2024
Accepted Date: July 10, 2024
Muftuoglu M, Andreeff M. Sample Multiplexing in CyTOF: Path to Optimize Single-cell Proteomic Profiling. Cell Signal. 2024;2(1):113-119.
Copyright: © 2024 Muftuoglu M, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Abstract
Sample multiplexing significantly enhanced the depth of single-cell proteomic analysis in CyTOF (Cytometry by Time-Of-Flight). New polymer-based chelators have broadened the utility of metal isotopes, enabling improved tagging and simultaneous analysis of multiple samples. These approaches minimize batch effects, streamline experiments, conserve valuable samples, reduce costs, enhance throughput, and increase the accuracy of biological data, thereby facilitating novel discoveries.
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