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Journal of Cancer Biology

ISSN: 2692-7896

Research Article Open Access
Volume 4 | Issue 2 | DOI: https://doi.org/10.46439/cancerbiology.4.054

Investigation of CBL-b, c-CBL expressions in colorectal cancer patients and their relationship with clinicopathological characteristics

Reyhaneh Yarmohammadi1,2, Minoo Pargol2, Zahra Mozooni3, Leyla Bahadorizadeh3,4,*, Sara Minaeian3, Shadi Tajvidi2
  • 1Doctoral Student, Carolina University, Winston-Salem, NC, USA
  • 2Skin and Stem Cell Research Center, Tehran University of Medical Sciences, Tehran, Iran
  • 3Antimicrobial Resistance Research Center, Institute of Immunology and Infectious Diseases, Iran University of Medical Sciences, Tehran, Iran
  • 4Department of Internal Medicine, Iran University of Medical Sciences, Tehran, Iran
+ Affiliations - Affiliations

Corresponding Author

Leyla Bahadorizadeh, L.bahadorizadeh@yahoo.com

Received Date: August 12, 2023

Accepted Date: November 03, 2023

Citation:

Yarmohammadi R, Pargol M, Mozooni Z, Bahadorizadeh L, Minaeian S, Tajvidi S. Investigation of CBL-b, c-CBL expressions in colorectal cancer patients and their relationship with clinicopathological characteristics. J Cancer Biol. 2023;4(2):42-48.


Copyright: © 2023 Yarmohammadi R, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

Introduction: Colorectal cancer (CRC) is the most common gastrointestinal cancer and a major cause of cancer-related mortality worldwide. Molecular indicators of pathogenic factors that regulate CRC, which are known as oncogenes, are desirable for response to treatment and improvement of CRC patient management. Several risk factors play a role in the development of this disease, which can occur genetically, familial, or sporadically. CRC tumorigenesis is stimulated by the proto-oncogene β-catenin (wnt/β-catenin). Casitas B-lineage lymphoma (c-Cbl) inhibits CRC tumor growth through an unknown mechanism that affects nuclear β-catenin. The current objective of this study is to evaluate the expression levels of Cbl-b and c-Cbl genes to determine if their transcripts can be used as suitable diagnostic indicators. Additionally, we aim to investigate the correlation between clinicopathological information of CRC patients and the levels of Cbl-b and c-Cbl.

Materials and Methods: Quantitative Real-Time PCR (qRT-PCR) method was used to evaluate the expression levels of Cbl-b and c-Cbl in 45 colorectal tumor tissues and 45 adjacent control tissues. Furthermore, we analyzed the diagnostic power of Cbl-b and c-Cbl by plotting receiver operating characteristic (ROC) curves.

Results: Our results showed that the expression levels of Cbl-b and c-Cbl were significantly increased in CRC patients compared to the adjacent control group (P<0.003, P<0.004). Analysis of clinicopathological features of CRC patients revealed that the expression of Cbl-b and c-Cbl was associated differently with TMN stage, LVI+ (P<0.0003, P<0.0001) (P<0.003, P<0.07).

Conclusion: These results indicate that the levels of Cbl-b and c-Cbl may serve as potential diagnostic indicators for CRC.

Keywords

Colorectal cancer (CRC), c-Cbl, Cbl-b, Diagnostic biomarkers, Real-time PCR (qRT-PCR)

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