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Research Article Open Access
Volume 1 | Issue 1 | DOI: https://doi.org/10.46439/signaling.1.001

EGCG modulates nuclear formaldehyde-induced Tau phosphorylation in neuronal cells

  • 1Neurobiology Group, Biochemical Sciences Division, CSIR-National Chemical Laboratory, Dr. Homi Bhabha Road, Pune-411008, India
  • 2Drosophila Neurobiology lab, National Centre for Cell Science, Pune-411007, India
  • 3The School of Bioengineering Sciences and Research, Maharasthra Institute of Technology, Loni Kalbhor, 412201, Pune, India
  • 4Academy of Scientific and Innovative Research (AcSIR), Pune-411008, India
+ Affiliations - Affiliations

Corresponding Author

Subashchandrabose Chinnathambi, s.chinnathambi@ncl.res.in

Received Date: October 25, 2022

Accepted Date: December 14, 2022

Abstract

Tau hyperphosphorylation is one of the major causes of Alzheimer’s disease pathology. The abnormal  phosphorylation curtails the physiological function of Tau of microtubule stabilization and renders  it more prone to aggregation. Apart from its function in the cytoplasm, Tau is attributed to play a  role in the nucleus. Nuclear function of Tau is dependent on its residue-specific phosphorylation. We studied the effect of a green tea polyphenol, EGCG, on the formaldehyde-induced Tau phosphorylation  and Tau kinase CDK5. Interestingly, we observed unique localization of phospho-Tau (AT8 and AT100) in the nucleus in various EGCG treatments. EGCG was also found to lower the levels of CDK5 in the formaldehyde-treated cells. Further, the role of EGCG was tested in vivo in drosophila eye model of hyperphosphorylated Tau (Tau E14). The results suggest that EGCG can modulate nuclear Tau phosphorylation and lower the levels of Tau kinase CDK5

Keywords

 Alzheimer’s disease, Nuclear Tau, Tau phosphorylation, CDK5, Drosophila model of Tau phosphorylation, EGCG

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