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Research Article Open Access
Volume 1 | Issue 1 | DOI: https://doi.org/10.46439/toxicology.1.003

Determination of Pregabalin in Tissues and Fluids by Using GC

  • 1Forensic Medicine Authority, Chemical Lab, Egypt
  • 2Faculty of Science, Mansoura, Egypt
+ Affiliations - Affiliations

Corresponding Author

Ahmed MA Shihata, ahmed_shihata_7@yahoo.com

Received Date: September 25, 2019

Accepted Date: November 20, 2019

Abstract

Pregabalin is an antiepileptic and analgesic drug, commercialized under the name of Lyrica and other names, generally used to treat neuropathic pain. The determination of pregabalin was quantified in tissues and fluids samples by using Gas chromatography coupled with mass spectrometry, isolation and precipitation protein by Ammonium Sulfate method. The limit of detection (LOD) is 200 ng and the limit of quantification (LOQ) in is 400 ng. Ibuprofen was used as internal standard, this methods has been applied in two cases of pregabalin.

Objective: To determine pregabalin in two cases study tissues and blood by using method isolation and precipitation of proteins by Ammonium Sulfate extraction, quantified by GC-MS.

Keywords

Pregabalin, GC-MS, Ammonium Sulfate, Human Tissues and Blood

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