Abstract
Pregabalin is an antiepileptic and analgesic drug, commercialized under the name of Lyrica and other names, generally used to treat neuropathic pain. The determination of pregabalin was quantified in tissues and fluids samples by using Gas chromatography coupled with mass spectrometry, isolation and precipitation protein by Ammonium Sulfate method. The limit of detection (LOD) is 200 ng and the limit of quantification (LOQ) in is 400 ng. Ibuprofen was used as internal standard, this methods has been applied in two cases of pregabalin.
Objective: To determine pregabalin in two cases study tissues and blood by using method isolation and precipitation of proteins by Ammonium Sulfate extraction, quantified by GC-MS.
Keywords
Pregabalin, GC-MS, Ammonium Sulfate, Human Tissues and Blood